Importance of homogenization in platelet-rich plasma researches

  • Ali Gokkaya Abant Izzet Baysal University, Medical School, Plastic Surgery, Bolu
  • Metin Gorgu Abant Izzet Baysal University, Medical School, Plastic Surgery
  • Ertuğrul Karanfil Abant Izzet Baysal University, Medcal School, Plastic Surgery, Bolu, Turkey
  • Jehat Kizilkan Abant Izzet Baysal University, Medcal School, Plastic Surgery, Bolu, Turkey
  • Ali Dogan Abant Izzet Baysal University, Medcal School, Plastic Surgery, Bolu, Turkey
Keywords: Platelet-rich plasma, platelet-poor plasma, homogenization

Abstract

Aim: To achieve homogenization, one of the most important factors to overcome during platelet-rich plasma (PRP) is standardization phases.

Method: We used 10 cc 'BD vacutainer ACD-A' tubes which consisted of 1.5 cc ACD-A. 3 tubes blood was collected from healthy volunteers from 20 to 40 years. All tubes were centrifuged by 180g x 12 min. Then 0.01 ml PRP was collected from just the level of buffy coat.

Results: Platelet concentration decreased from buffy coat to top of PPP as expected. If any counting done before homogenization the results will be misleading.

Conclusion: When we sample 3 cc PRP homogenized with shaker at 3 point from 3 different point, we see that they are close to each other. It is important that the PRP to be used in the studies must be homogenized before any measurement.

Published
2018-03-16