Alginate encapsulation induce colony formation with umbilical cord-derived mesenchymal stem cells

  • Erkan Gumus Department of Histology and Embryology, Aydin Adnan Menderes University, School of Medicine, Aydin, Turkey http://orcid.org/0000-0001-6432-7457
  • Burcin Irem Abas Department of Biochemistry, Aydin Adnan Menderes University, School of Medicine, Aydin, Turkey http://orcid.org/0000-0002-1018-5577
  • Evrim Cevik Department of Machinery and Metal Technologies, Aydin Adnan Menderes University, Kocarli Vocational High School, Aydin, Turkey http://orcid.org/0000-0003-3286-4367
  • Bilge Kocabiyik Department of Molecular Biotechnology, Aydin Adnan Menderes University, Graduate School of Health Sciences, Aydin, Turkey http://orcid.org/0000-0002-9687-5260
  • Melike Cenik Department of Molecular Biotechnology, Aydin Adnan Menderes University, Graduate School of Health Sciences, Aydin, Turkey http://orcid.org/0000-0002-5134-6082
  • Ozge Cevik Department of Biochemistry, Aydin Adnan Menderes University, School of Medicine, Aydin, Turkey http://orcid.org/0000-0002-9325-3757
Keywords: Alginate, hydrogel, mesenchymal stem cell, umbilical cord

Abstract

Aim: The umbilical cord (UC) is a rich source of mesenchymal stem cell (MSC) isolation. Since the MSCs isolated from here have high self-renewal capacity and differentiation potential, production through biofabrication is essential for clinical treatments. For the cells to be stored for a long time and presented ready for use, encapsulation is required. In this study, UC-MSC cells were encapsulated with alginate using three different methods: alginate drop, alginate coating, and alginate sphere.

Methods: The cell viability, live/dead cell ratio, and colony formation capacities of the encapsulated cells were examined for 14 days.

Results: In the study, it was found that the most effective method was the alginate sphere form and that the structure of the cells should be preserved by injecting them into biomaterials in encapsulation. Colony formation potential was found to be high in biomaterials with alginate spheres.

Conclusion: As a result, the preservation of UC-MSC cells with alginate sphere encapsulation via biofabrication and their clinical use availability may be beneficial for treating of many diseases.

Published
2021-03-27
How to Cite
Gumus, E., Abas, B., Cevik, E., Kocabiyik, B., Cenik, M., & Cevik, O. (2021, March 27). Alginate encapsulation induce colony formation with umbilical cord-derived mesenchymal stem cells. EXPERIMENTAL BIOMEDICAL RESEARCH, 4(2), 113-121. https://doi.org/https://doi.org/10.30714/j-ebr.2021267974