Radiolabeling and quality control studies of [99mTc]Tc-dexketoprofen trometamol for inflammation imaging

Abstract

Aim: To radiolabel dexketoprofen trometamol (DT) with [^99mTc]Tc for developing inflammation imaging agent.

Method: For this purpose, DT was radiolabeled using [^99mTc]Tc, and radioactive thin layer chromatography (RTLC) was used in the quality control studies of [^99mTc]Tc-DT. In addition, the effects of quality control factors (reducing agent, incubation time, pH value) on radiolabeling were studied. Finally, the stability study of [^99mTc]Tc-DT was performed and the partition coefficient value of [^99mTc]Tc-DT was calculated.

Results: Based on the results, a new, simple, and fast direct technique was used for producing [^99mTc]Tc-DT with over 92% labeling efficiency after 15 minutes of pH 9.0 incubation. 37 MBq [^99mTc]Tc, 10 µg of stannous chloride dihydrate (reducing agent), and 250 µg of DT were utilized to produce the ideal radiolabeling conditions. According to the RTLC experiments, [^99mTc]Tc-DT remained stable at room temperature for six hours. The [^99mTc]Tc-DT logP has been determined to be -0.48 ± 0.02.

Conclusions: Based on the acquired results, [^99mTc]Tc-DT has the potential to be an imaging agent for inflammation. Further studies are in progress, such as biodistribution of [^99mTc]Tc-DT.