Anti-proliferative effects of salmon calcitonin on SH-SY5Y neuroblastoma in vitro

Abstract

Aim: We aimed to examine the potential cytotoxic effect of salmon calcitonin, which is one of the components that regulates mineral metabolism and prevents the increase in the amount of calcium, on SH-SY5Y cells, a neuroblastoma cell line.

Methods: SH-SY5Y cells were cultured in DMEM medium in the presence of 37°C and 5% CO₂ in conventional culture flasks. MTT assay was applied to investigate the effect of calcitonin individually on SH-SY5Y cells by treatment different concentrations for 24 h and performed.

Results: In cells cultured with salmon calcitonin applied at different concentrations (0.1, 1, 3.125, 6,25, 12.5, 25, 50 and 100 nM/ml), anti-proliferation was statistically significant at concentrations of 50 and 100 nM/ml compared to the control group. It showed that 50 nM/ml and 100 nM/ml had the highest cytotoxic effect on SH-SY5Y for 24 h

Conclusions: Considering the proliferation curve of SH-SY5Y, the results show that salmon calcitonin treatment potentiated the proliferative activities by inhibiting cell viability in SH-SY5Y cells at concentrations of 50 and 100 nM/ml. Further studies exploring salmon calcitonin’s protective effects may prove successful and maybe it is a promising agent for cancer treatment.