Importance of homogenization in platelet-rich plasma researches

Authors

  • Ali Gokkaya Abant Izzet Baysal University, Medical School, Plastic Surgery, Bolu
  • Metin Gorgu Abant Izzet Baysal University, Medical School, Plastic Surgery
  • Ertuğrul Karanfil Abant Izzet Baysal University, Medcal School, Plastic Surgery, Bolu, Turkey
  • Jehat Kizilkan Abant Izzet Baysal University, Medcal School, Plastic Surgery, Bolu, Turkey
  • Ali Dogan Abant Izzet Baysal University, Medcal School, Plastic Surgery, Bolu, Turkey

DOI:

https://doi.org/10.30714/j-ebr.2018237494

Keywords:

Platelet-rich plasma, platelet-poor plasma, homogenization

Abstract

Aim: To achieve homogenization, one of the most important factors to overcome during platelet-rich plasma (PRP) is standardization phases.

Method: We used 10 cc 'BD vacutainer ACD-A' tubes which consisted of 1.5 cc ACD-A. 3 tubes blood was collected from healthy volunteers from 20 to 40 years. All tubes were centrifuged by 180g x 12 min. Then 0.01 ml PRP was collected from just the level of buffy coat.

Results: Platelet concentration decreased from buffy coat to top of PPP as expected. If any counting done before homogenization the results will be misleading.

Conclusion: When we sample 3 cc PRP homogenized with shaker at 3 point from 3 different point, we see that they are close to each other. It is important that the PRP to be used in the studies must be homogenized before any measurement.

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Published

2018-03-16

How to Cite

Gokkaya, A., Gorgu, M., Karanfil, E., Kizilkan, J., & Dogan, A. (2018). Importance of homogenization in platelet-rich plasma researches. EXPERIMENTAL BIOMEDICAL RESEARCH, 1(2), 32–36. https://doi.org/10.30714/j-ebr.2018237494

Issue

Vol. 1 No. 2 (2018): Experimental Biomedical Research

Section

Articles

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