Effects of magnesium sulphate on liver ischemia/reperfusion injury in a rat model

  • Kerem Akkoca Department of Anesthesia and Reanimation, Bolu Abant Izzet Baysal University, Faculty of Medicine, Bolu, Turkey http://orcid.org/0000-0003-4154-8606
  • Hamit Yoldas Department of Anesthesia and Reanimation, Bolu Abant Izzet Baysal University, Faculty of Medicine, Bolu, Turkey http://orcid.org/0000-0002-9265-1008
  • Mustafa Sit Department of General Surgery, Bolu Abant Izzet Baysal University, Faculty of Medicine, Bolu, Turkey http://orcid.org/0000-0002-7475-7298
  • Ibrahim Karagoz Department of Anesthesia and Reanimation, Bolu Abant Izzet Baysal University, Faculty of Medicine, Bolu, Turkey http://orcid.org/0000-0003-2954-4784
  • Isa Yildiz Department of Anesthesia and Reanimation, Bolu Abant Izzet Baysal University, Faculty of Medicine, Bolu, Turkey http://orcid.org/0000-0001-7476-2217
  • Abdullah Demirhan Department of Anesthesia and Reanimation, Bolu Abant Izzet Baysal University, Faculty of Medicine, Bolu, Turkey http://orcid.org/0000-0002-2311-6431
  • Murat Bilgi Department of Anesthesia and Reanimation, Bolu Abant Izzet Baysal University, Faculty of Medicine, Bolu, Turkey http://orcid.org/0000-0002-9001-2309
  • Ozgur Mehmet Yis Department of Biochemistry, Bolu Abant Izzet Baysal University, Faculty of Medicine, Bolu, Turkey http://orcid.org/0000-0002-7006-3125
  • Aysel Kukner Department of Histology and Embryology, Near East University, Faculty of Medicine, Nicosia, TRNC http://orcid.org/0000-0001-5387-2628
  • Ayhan Cetinkaya Department of Physiology, Bolu Abant Izzet Baysal University, Faculty of Medicine, Bolu, Turkey http://orcid.org/0000-0002-8212-7149
  • Oguz Catal Department of General Surgery, Bolu Abant Izzet Baysal University, Faculty of Medicine, Bolu, Turkey http://orcid.org/0000-0002-4067-251X
  • Bahri Ozer Department of General Surgery, Bolu Abant Izzet Baysal University, Faculty of Medicine, Bolu, Turkey http://orcid.org/0000-0002-4326-2102
Keywords: Liver ischemia/reperfusion injury, magnesium sulfate, oxidative stress, rat

Abstract

Aim: To investigate the protective efficacy of magnesium sulphate in a model of rat liver ischemia-reperfusion (I/R) injury.

Method: 32 adult female Wistar-Albino rats (250 to 350 g) were used in this experimental study. Rats were divided into 4 groups according to liver ischemia and magnesium sulfate application methods. Group 1 (C); control, group 2 (M); magnesium sulphate, group 3 (I/R); liver I/R, group 4 (I/R+M); I/R + magnesium sulphate treated. The blood samples were centrifuged for the study of aspartate aminotransferase (AST), alanine aminotransferase, prothrombin time (PT), international normalized ratio (INR) troponin I, total antioxidant status (TAS), total oxidant status (TOS) assays. The livers of the animals were removed at the end of the study and samples were taken for histopathological examination.

Results: AST and INR values were significantly decreased in I/R+M group compared to I/R group. There was no significant difference in ALT values of the groups. Although not statistically significant, the TAS values were increased in I/R + M group compared to I/R group rats. In addition, the value of TOS was found to be lower in I/R + M group rats. In the histopathological examination, the mean values of apoptosis and necrosis were lower in the IR+M group compared to the IR group.

Conclusion: The main finding of the present study suggested that magnesium sulphate pretreatment moderately decreased the liver damage through its anti-inflammatory and anti-oxidant effects in a rat model of liver I/R.

Published
2019-06-30