Use of salidroside in a lipopolysaccharide-induced periventricular leukomalacia model

  • Mustafa Dilek Bolu Abant Izzet Baysal University, Faculty of Medicine, Department of Pediatrics, Bolu, Turkey
  • Gokce Kaya Dincel Bolu Abant Izzet Baysal University, Faculty of Medicine, Department of Pediatrics, Bolu, Turkey
  • Ayhan Cetinkaya Bolu Abant Izzet Baysal University, Experimental Animal Application and Research Center, Bolu, Turkey
  • Mervan Bekdas Bolu Abant Izzet Baysal University, Faculty of Medicine, Department of Pediatrics, Bolu, Turkey
  • Nimet Kabakus Bolu Abant Izzet Baysal University, Faculty of Medicine, Department of Pediatrics, Bolu, Turkey
Keywords: Salidroside;, lipopolysaccharide;, periventricular leukomalacia, rat model, neuroprotection.

Abstract

Aim: Research into the different treatment methods based on the intrauterine lipopolysaccharide (LPS)-induced Periventricular leukomalacia (PVL) model, as one of the main causes of morbidity in preterm infants still continues to be relevant. The present study investigates the effect on PVL of salidroside obtained from Rhodiola Rosea (golden root, orpin rose), which is a plant with known for its medicinal qualities.

Method: To develop an induced PVL model, a 500 microgram/kg dose of LPS (Escherichia coli, serotype 055:B5, Sigma) was applied to two pregnant rats intraperitoneally on day 18, day 19 and day 20 of gestation. One of the LP applied rats was given 25 mg/kg Salidroside (250 mg Rhodiola root extract capsules, which include 3 mg Salidroside) by oral gavage (LPS+Salidroside), and a physiological saline solution was given to the control group. After delivery, 10 offspring of the LPS-applied mother, nine offspring of the LPS+Salidrosideapplied mother and seven offspring of the control mother were sacrificed on postnatal Day 7 with ether anesthesia. The caspase enzyme located in apoptosis pathways of 10 percent neutral-buffered formalin fixed brain tissue was stained immunohistochemically, and apoptotic cells were counted.

Results: No statistically significant difference was noted between the LPS+Salidroside group and the control group, while a statistically significant difference was noted between the LPS and LPS+Salidroside groups. It was observed that Salidroside reduced LPS induced apoptosis.

Conclusion: The intended experimental neuroprotective effect of Salidroside usage was provided through the inhibition of apoptosis in a PVL-damaged brain.

Published
2018-09-24