Investigation of the effect of REM sleep deprivation on epileptic seizures caused by pentylenetetrazole in mice

  • Recep Akkaya Department of Biophysics, Sivas Cumhuriyet University, School of Medicine, Sivas, Turkey http://orcid.org/0000-0002-3477-7198
  • Sebahattin Karabulut Department of Medical Services and Techniques, Sivas Cumhuriyet University, Vocational School of Health Services, Sivas, Turkey http://orcid.org/0000-0002-3261-4125
  • Ahmet Sevki Taskiran Department of Medical Physiology, Sivas Cumhuriyet University, School of Medicine, Sivas, Turkey http://orcid.org/0000-0002-5810-8415
Keywords: REM sleep deprivation, hippocampus, seizure susceptibility, oxidative stress

Abstract

Aim: To investigate whether different periods of rapid eye movement sleep deprivation (REM SD) contribute to seizure susceptibility, hippocampal oxidative status and balance of inhibition-excitation in the acute epilepsy model.

Methods: REM SD was performed using the modified multiple platforms method on adult male BALB/c mice. Pentylentetrazol (PTZ) was injected to induce seizures and hippocampal total antioxidant status (TAS), total oxidant status (TOS), gamma aminobutyric acid (GABA), and glutamate levels were measured using the ELISA method.

Results: PTZ-induced seizures following 8 h and 72 h REM SD significantly reduced the hippocampal TAS levels, but did not affect the TOS levels. In REM SD groups, especially after 8 hours of REM sleep loss, there was a significant increase in glutamate in PTZ induction. The hippocampal GABA levels were increased by PTZ-induced seizures after 72 h REM SD. PTZ- induction after 8 hours of RAM SD leads to a significant increase in the seizure duration.

Conclusion: It can be speculated that the REM SD can contribute to seizure susceptibility by changing the oxidant-antioxidant balance and excitatory and inhibitory tone in the hippocampus.

Published
2020-06-13
How to Cite
Akkaya, R., Karabulut, S., & Taskiran, A. (2020, June 13). Investigation of the effect of REM sleep deprivation on epileptic seizures caused by pentylenetetrazole in mice. EXPERIMENTAL BIOMEDICAL RESEARCH, 3(3), 149-156. https://doi.org/https://doi.org/10.30714/j-ebr.2020361050